Diffusion-Induced Hydrophilic Conversion of Polydimethylsiloxane/Block-Type Phospholipid Polymer Hybrid Substrate for Temporal Cell-Adhesive Surface

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• 作者：Ji-Hun Seo ; Kazuhiko Ishihara
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：August 24, 2016
• 年：2016
• 卷：8
• 期：33
• 页码：21839-21846
• 全文大小：437K
• 年卷期：0
• ISSN：1944-8252

文摘

In this study, diffusion-induced hydrophobic–hydrophilic conversion of the surface of the cross-linked polydimethylsiloxane (PDMS) substrate was realized by employing a simple swelling–deswelling process of PDMS substrate in a block-type polymer solution with the aim of development of a temporal cell-adhesive substrate. The ABA block-type polymer composed of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) segment and PDMS segment with over 70% of dimethylsiloxane unit composition could be successfully incorporated in the PDMS substrate during the swelling–deswelling process to prepare the PDMS/phospholipid block copolymer hybrid substrates. During the aging process of the PDMS substrate for 4 days in aqueous medium, its surface property changed gradually from hydrophobic to hydrophilic. X-ray photoelectron spectroscopy and atomic force microscopy data provided strong evidence that the time-dependent hydrophilic conversion of the PDMS/block-type phospholipid polymer hybrid substrate was induced by the diffusion of the hydrophilic PMPC segment in the block-type polymer to be tethered on the substrate. During the hydrophilic conversion process, surface-adsorbed fibronectin was gradually desorbed from the substrate surface, and this resulted in successful detachment of two-dimensional connected cell crowds.