Caspase Sensitive Gold Nanoparticle for Apoptosis Imaging in Live Cells

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• 作者：In-Cheol Sun ; Seulki Lee ; Heebeom Koo ; Ick Chan Kwon ; Kuiwon Choi ; Cheol-Hee Ahn ; Kwangmeyung Kim
• 刊名：Bioconjugate Chemistry
• 出版年：2010
• 出版时间：November 17, 2010
• 年：2010
• 卷：21
• 期：11
• 页码：1939-1942
• 全文大小：226K
• 年卷期：v.21,no.11(November 17, 2010)
• ISSN：1520-4812

文摘

We developed a new apoptosis imaging probe with gold nanoparticles (AuNPs). A near-infrared fluorescence dye was attached to AuNP surface through the bridge of peptide substrate (DEVD). The fluorescence was quenched in physiological conditions due to the quenching effect of AuNP, and the quenched fluorescence was recovered after the DEVD had been cleaved by caspase-3, the enzyme involved in apoptotic process. The adhesion of DEVD substrates on AuNP surface was accomplished by conjugation of the 3,4-dihydroxy phenylalanine (DOPA) groups which are adhesive to inorganic surface and rich in mussels. This surface modification with DEVD substrates by DOPA groups resulted in increased stability of AuNP in cytosol condition for hours. Moreover, the cleavage of substrate and the dequenching process are very fast, and the cells did not need to be fixed for imaging. Therefore, the real-time monitoring of caspase activity could be achieved in live cells, which enabled early detection of apoptosis compared to a conventional apoptosis kit such as Annexin V-FITC. Therefore, our apoptosis imaging has great potential as a simple, inexpensive, and efficient apoptosis imaging probe for biomedical applications.