Selection of Streptavidin Binders from a DNA-Encoded Chemical Library

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• 作者：Christoph E. Dumelin ; Jö ; rg Scheuermann ; Samu Melkko ; and Dario Neri
• 刊名：Bioconjugate Chemistry
• 出版年：2006
• 出版时间：March 2006
• 年：2006
• 卷：17
• 期：2
• 页码：366 - 370
• 全文大小：234K
• 年卷期：v.17,no.2(March 2006)
• ISSN：1520-4812

文摘

DNA-encoded libraries of small organic molecules facilitate the construction of large, encoded self-assemblingchemical libraries for the identification of high-affinity binders to protein targets. We have constructed a libraryof 477 chemical compounds, coupled to 48mer-oligonucleotides, each containing a unique six-base sequenceserving as "bar-code" for the identification of the chemical moiety. The functionality of the library was confirmedby selection and amplification of both high- and low-affinity binding molecules specific to streptavidin.