Determination of 10 Carcinogenic Polycyclic Aromatic Hydrocarbons in Mainstream Cigarette Smoke
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- 作者：Yan S. Ding ; David L. Ashley ; Clifford H. Watson
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：July 25, 2007
- 年：2007
- 卷：55
- 期：15
- 页码：5966 - 5973
- 全文大小：111K
- 年卷期：v.55,no.15(July 25, 2007)
- ISSN：1520-5118
文摘
Polycyclic aromatic hydrocarbons (PAHs) are one class of chemical compounds that (1) are presentat low to trace levels in unburned cigarette filler, and (2) are predominantly generated duringcombustion. According to a recent report of the International Agency for Research on Cancer, 10carcinogenic PAHs together with 53 other known carcinogens are present in cigarette smoke. Accuratequantification of these chemicals helps assess public health risk to both smokers and nonsmokersexposed to second-hand smoke. We have developed and validated a specific and sensitive methodfor measuring these 10 carcinogenic PAHs in the particulate phase of mainstream tobacco smoke.Cigarette smoke particulate, produced using standard machine smoking protocols, was collected onglass fiber Cambridge filter pads. The particulate matter was solvent extracted, purified by solid-phase extraction, and analyzed by liquid chromatography/atmospheric pressure photoionizationtandem mass spectrometry using isotopically labeled analogues as internal standards. Our method'slimits of detection ranged from 11 to 166 pg and achieved sufficient reproducibility and accuracy toprovide useful information on a range of cigarettes having dramatically different machine-smoked tarand nicotine deliveries. The identity of each PAH analyte was established from chromatographicretention time, analyte-specific fragmentation patterns, and relative peak area ratios of the product/precursor ion pairs. This new method provides higher sensitivity, specificity, and throughput than didearlier methods. We found relatively consistent PAH levels among a selection of domestic full-flavorcigarettes. The PAH levels in smoke from highly ventilated light and ultralight cigarettes were lowwhen smoked using ISO (International Organization for Standardization) conditions. However, if highlyventilated cigarettes were smoked under more intense conditions (e.g., larger or more frequent puffs,vents blocked), their PAH levels equaled or exceeded their full-flavor counterparts under ISOconditions.