Smart Hydrogel Particles: Biomarker Harvesting: One-Step Affinity Purification, Size Exclusion, and Protection against Degradation
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- 作者：Alessandra Luchini ; David H. Geho ; Barney Bishop ; Duy Tran ; Cassandra Xia ; Robert L. Dufour ; Clinton D. Jones ; Virginia Espina ; Alexis Patanarut ; Weidong Zhou ; Mark M. Ross ; Alessandra Tessitore ; Eman
- 刊名：Nano Letters
- 出版年：2008
- 出版时间：January 2008
- 年：2008
- 卷：8
- 期：1
- 页码：350 - 361
- 全文大小：458K
- 年卷期：v.8,no.1(January 2008)
- ISSN：1530-6992
文摘
Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such asalbumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independentfunctions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and(c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.