SFE-GC with Quantitative Transfer of the Extraction Effluent to a Megabore Capillary Column

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• 作者：Mark A. Stone and Larry T. Taylor
• 刊名：Analytical Chemistry
• 出版年：2000
• 出版时间：July 15, 2000
• 年：2000
• 卷：72
• 期：14
• 页码：3085 - 3092
• 全文大小：118K
• 年卷期：v.72,no.14(July 15, 2000)
• ISSN：1520-6882

文摘

Supercritical fluid extraction was directly coupled with gaschromatography such that the entire extraction effluentwas directed onto the GC column. A sealed connectionbetween the restrictor and the column ensured that noloss of analyte could occur. The elevated pressure generated in the column itself resulted in a reduced flow ratethrough the column, making efficient trapping possible.Metal-jacketed capillary columns were used as they cantolerate the elevated pressures required. In addition tomaximizing sensitivity, very practical extraction parameters were possible. For a 25-min extraction, with a 2.2mL/min flow rate, and the column at 40 C, analytes asvolatile as C-14 were trapped and separated. Trappingefficiency improved further with shorter extraction times,mild cooling of the column, or lower flow rates.