Global Comparison of the Membrane Subproteomes between a Multidrug-Resistant Acinetobacter baumannii Strain and a Reference Strain

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• 作者：Axel Siroy ; Pascal Cosette ; Damien Seyer ; Christelle Lemaî ; tre-Guillier ; David Vallenet ; Alain Van Dorsselaer ; Sophie Boyer-Mariotte ; Thierry Jouenne ; Emmanuelle Dé
• 刊名：Journal of Proteome Research
• 出版年：2006
• 出版时间：December 2006
• 年：2006
• 卷：5
• 期：12
• 页码：3385 - 3398
• 全文大小：494K
• 年卷期：v.5,no.12(December 2006)
• ISSN：1535-3907

文摘

Acinetobacter baumannii causes severe infections in compromised patients. We combined SDS-PAGE,two-dimensional gel electrophoresis and mass spectrometry (LC-MS/MS and MALDI-TOF) to separateand characterize the proteins of the cell envelope of this bacterium. In total, 135 proteins (inner andouter membrane proteins) were identified. In this analysis, we described the expression by this bacteriumof RND-type efflux systems and some potential virulence factors. We then compared the membranesubproteome of a clinical multidrug-resistant (MDR) isolate with that of a reference strain. We foundthat the MDR strain expressed lower levels of the penicillin-binding-protein 1b, produced a CarO proteinhaving different primary and quaternary structures to that of the reference strain, and expressed OmpWisoforms. We also showed that the clinical strain has a high ability to form biofilms consistent with theaccumulation of some outer membrane proteins (OMPs) such as NlpE or CsuD that have already beendescribed as involved in bacterial adhesion. These features may partly explain the MDR emergence ofthe clinical isolate.