Polysaccharide from Ganoderma atrum Evokes Antitumor Activity via Toll-like Receptor 4-Mediated NF-魏B and Mitogen-Activated Protein Kinase Signaling Pathways
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- 作者：Shenshen Zhang ; Shaoping Nie ; Danfei Huang ; Jianqin Huang ; Yawei Wang ; Mingyong Xie
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：April 17, 2013
- 年：2013
- 卷：61
- 期：15
- 页码：3676-3682
- 全文大小：281K
- 年卷期：v.61,no.15(April 17, 2013)
- ISSN：1520-5118
文摘
Ganoderma atrum has been used as a traditional Chinese medicine for centuries. In this study, the antitumor activity of a novel G. atrum polysaccharide (PSG-1) was investigated in vitro and in vivo using S180 tumor-bearing mice. The results showed that PSG-1 significantly inhibited the proliferation of S180 via the activation of macrophages in a dose-dependent manner. PSG-1-primed macrophages exhibited a higher tumoricidal activity than untreated macrophages. Administration of PSG-1 significantly inhibited the growth of transplantable sarcoma S180-bearing mice and increased macrophage phagocytosis and the levels of cytokines and nitride oxide. Expression of Toll-like receptor (TLR) 4 in the membrane was markedly increased in PSG-1-treated groups, suggesting that it may be a possible receptor for PSG-1. PSG-1 also promoted the translocation of the p65 subunit of NF-魏B from cytosol to nucleus and the degradation of I魏B伪. Moreover, the phosphorylation of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases 1/2, and c-Jun N-terminal kinase in macrophages was improved by PSG-1 in a dose-dependent manner. Therefore, it is suggested that PSG-1 may elicit its antitumor effect by improving immune system functions through TLR4-mediated NF-魏B and MAPK signaling pathways.