Lead Analysis by Anti-Chelate Fluorescence Polarization Immunoassay
详细信息 查看全文
- 作者：David K. Johnson ; Sherry M. Combs ; John D. Parsen ; and Michael E. Jolley
- 刊名：Environmental Science & Technology
- 出版年：2002
- 出版时间：March 1, 2002
- 年：2002
- 卷：36
- 期：5
- 页码：1042 - 1047
- 全文大小：66K
- 年卷期：v.36,no.5(March 1, 2002)
- ISSN：1520-5851
文摘
Lead concentrations were determined by a fluorescencepolarization immunoassay (FPIA) method that uses polyclonalantibodies raised against the lead(II) chelate of ethylenediamine-N,N,N',N'-tetraacetic acid (EDTA). The techniqueis based on competition for a fixed concentration of antibodybinding sites between Pb-EDTA, formed by treating thesample with excess EDTA, and a fixed concentration of afluorescent analogue of the Pb-EDTA complex. Theobjective was to correlate results obtained by FPIA withthose produced by conventional atomic spectroscopy analysisof soils, solid waste leachates (produced by the ToxicityCharacteristic Leachate Procedure; TCLP), airborne dust,and drinking water. Linear regression analysis of FPIA resultsfor 138 soil samples containing 0-3094 ppm Pb(II) byflame atomic absorption spectroscopy and 40 TCLP extractscontaining 0-668 ppm Pb(II) by inductively coupledplasma atomic emission spectroscopy produced correlationcoefficients (r2) of 0.96 and 0.93, respectively. Pilotstudies of mineral acid extracts of airborne dust trappedon fiberglass filters and of two sources of drinking waterdemonstrated the feasibility of also measuring lead inthese matrixes by FPIA. The limit of detection under conditionsthat minimized sample dilution was approximately 1 ppb,and cross reactivity with 15 nontarget metals was below0.5% in all cases. The methods are simple to perform and areamenable to field testing and mobile laboratory use,allowing timely and cost-effective characterization ofsuspected sources of lead contamination.