Rapid and Ratiometric Fluorescent Detection of Cysteine with High Selectivity and Sensitivity by a Simple and Readily Available Probe
详细信息 查看全文
- 作者：Yao Liu ; Dehuan Yu ; Shuangshuang Ding ; Qi Xiao ; Jun Guo ; Guoqiang Feng
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2014
- 出版时间：October 22, 2014
- 年：2014
- 卷：6
- 期：20
- 页码：17543-17550
- 全文大小：433K
- ISSN：1944-8252
文摘
We report a simple and readily available fluorescent probe for rapid, specific, and ratiometric fluorescent detection of the biologically important cysteine (Cys). This probe uses a visible-light excitable excited-state intramolecular proton transfer (ESIPT) dye (4鈥?dimethylamino-3-hydroxyflavone) as the fluorophore and an acrylate group as the ESIPT blocking agent as well as the recognition unit. Cleavage of the acrylate moiety can be achieved specifically and rapidly by Cys in aqueous solution under mild conditions, which leads to restore the ESIPT process and enables the probe to show a rapid, ratiometric fluorescent detection process for Cys with high selectivity over various analytes, including homocysteine (Hcy) and glutathione (GSH). The detection limit of this probe for Cys was found to be 鈭?.2 渭M and bioimaging of intracellular Cys by this probe was successfully applied in living cells, indicating that this probe holds great potential for biological applications.