Luminescence Lifetime Standards for the Nanosecond to Microsecond Range and Oxygen Quenching of Ruthenium(II) Complexes

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• 作者：Kaleem J. Morris ; Michael S. Roach ; Wenying Xu ; J. N. Demas ; B. A. DeGraff
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：December 15, 2007
• 年：2007
• 卷：79
• 期：24
• 页码：9310 - 9314
• 全文大小：187K
• 年卷期：v.79,no.24(December 15, 2007)
• ISSN：1520-6882

文摘

A rapid and reproducible method for determining thetemperature dependence of luminescence lifetimes hasbeen developed. With the use of this method, a set ofstandards for the excited-state lifetime oxygen quenchingof several ruthenium(II) transition metal complexes wasestablished. With the use of three solvents of differentviscosities and two metal complexes with widely differentlifetimes, an overlapping range of ca. 100 ns to 6 s wasobtained. The decays are pure single exponentials, whichmeans that they can be used reliably with both phase andpulsed lifetime instruments. For a pure single-exponentialdecay, a properly operating phase shift instrument willgive the same lifetime as a time domain instrument. Withthe use of a thermal deactivation model and a three-parameter temperature-dependent oxygen quenching constant, the lifetime temperature-dependent data was wellfit by a simple six-parameter equation that covers thetemperature range of 10-50 C and oxygen pressuresfrom 0 to 1 atm of oxygen with excellent precision (ca.<1%). This permits both laboratory and field calibrationof instruments.