Build-up of Collagen and Hyaluronic Acid Polyelectrolyte Multilayers

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• 作者：Jan Å ; . Johansson ; Tobias Halthur ; Merja Herranen ; Lennart Sö ; derberg ; Ulla Elofsson ; and Jö ; ns Hilborn
• 刊名：Biomacromolecules
• 出版年：2005
• 出版时间：May 2005
• 年：2005
• 卷：6
• 期：3
• 页码：1353 - 1359
• 全文大小：223K
• 年卷期：v.6,no.3(May 2005)
• ISSN：1526-4602

文摘

We have used a novel polyelectrolyte multilayer (PEM) coating consisting of the polyelectrolytes collagenand hyaluronic acid. The build-up by the layer-by-layer deposition technique is outlined by ex situ and insitu ellipsometric measurements. When collagen was added, the thickness of the PEM was increased, andthe refractive index was decreased. Corresponding but opposite effects were noted when hyaluronic acidwas added. These changes are considered to be explained by a diffusion mechanism. It was also found thatthe PEM layers were unstable at physiological pH. However, by cross-linking using N-(3-di-methylaminopropyl)-N'-ethylcarbodiimide together with N-hydroxysuccinimide, a stable PEM layer resulted. Thesetissue friendly PEM layers are expected to have a great impact in the design of artificial extracellular matrixes.Also, the insertion of fluorescence labels demonstrates the potential for incorporation of other functionalities.