Establishing a Leaf Proteome Reference Map for Ginkgo biloba Provides Insight into Potential Ethnobotanical Uses
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- 作者：Lubica Uvackova ; Emilia Ondruskova ; Maksym Danchenko ; Ludovit Skultety ; J谩n A. Miernyk ; Pavel Hrub铆k ; Martin Hajduch
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2014
- 出版时间：November 26, 2014
- 年：2014
- 卷：62
- 期：47
- 页码：11547-11556
- 全文大小：311K
- ISSN：1520-5118
文摘
Although ginkgo (Maidenhair tree, Ginkgo biloba L.) is an ancient medicinal and ornamental tree, there has not previously been any systematic proteomic study of the leaves. Herein we describe results from the initial study identifying abundant ginkgo leaf proteins and present a gel reference map. Proteins were isolated from fully developed mature leaves in biological triplicate and analyzed by two-dimensional electrophoresis plus tandem mass spectrometry. Using this approach, we were able to reproducibly quantify 190 abundant protein spots, from which 157 proteins were identified. Most of identified proteins are associated with the energy and protein destination/storage categories. The reference map provides a basis for understanding the accumulation of flavonoids and other phenolic compounds in mature leaves (e.g., identification of chalcone synthase, the first committed enzyme in flavonoid biosynthesis). We additionally detected several proteins of as yet unknown function. These proteins comprise a pool of potential targets that might be useful in nontraditional medical applications.