We evaluated the differentially expressed proteins in the plasma of ovarian
can
cer (OVC) patients using2-D SDS-polya
crylamide gel ele
ctrophoresis (SDS-PAGE) with post-translational modifi
cation (PTM)spe
cifi
c stains after the removal of six high-abundan
ce proteins. The pooled plasma from patients withstage III or IV OVC was
compared to a pooled postmenopausal age-mat
ched
control. Several proteinswere identified as differentially expressed in the plasma of OVC patients. Among them, the phosphorylated fibrinogen-
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chain isoform (
containing fibrinopeptide-A) was found to be up-regulated.Previously in our laboratory, phosphorylated fibrinopeptide-A was found to be up-regulated in the lowmole
cular weight fra
ction of serum derived from OVC patients. We examined the levels of phosphorylated fibrinogen-
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chain in ea
ch patient that
constituted the pooled plasma using
Western blot,mass spe
ctrometry (MS), and PTM spe
cifi
c stains. Phosphoprotein bands
containing fibrinogen-
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chainfragments showed up-regulation in all OVC patients.