Production of Epigallocatechin Gallate 7-O--D-Glucopyranoside (EGCG-G1) Using the Glucosyltransferase from Leuconostoc mesenteroides

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• 作者：Eun-Kyung Hyun ; Ha-Young Park ; Hye-Jung Kim ; Jung-Kul Lee ; Doman Kim ; Deok-Kun Oh
• 刊名：Biotechnology Progress
• 出版年：2007
• 出版时间：October 2007
• 年：2007
• 卷：23
• 期：5
• 页码：1082 - 1086
• 全文大小：75K
• 年卷期：v.23,no.5(October 2007)
• ISSN：1520-6033

文摘

Epigallocatechin gallate 7-O--D-glucopyranoside (EGCG-G1), as a novel compound with ahigher water solubility and stability and similar antioxidant effect when compared withepigallocatechin gallate (EGCG), was produced from sucrose as a glucose donor and EGCG asa glucose acceptor by a glucosyltransferase from Leuconostoc mesenteroides. The pH andtemperature for maximum EGCG-G1 production by the glucosyltransferase were 5.0 and 25C, respectively. The enzyme and substrates concentrations to produce maximum EGCG-G1were 3.78 units/mL, 25 mM sucrose, and 1.5 mM EGCG, respectively. Under these conditions,the glucosyltransferase produced 1.0 mM EGCG-G1 from 1.5 mM EGCG at a reaction time of180 min, corresponding to formation of 67% product on a mole basis.