文摘
Epigallocatechin gallate 7-O--D-glucopyranoside (EGCG-G1), as a novel compound with ahigher water solubility and stability and similar antioxidant effect when compared withepigallocatechin gallate (EGCG), was produced from sucrose as a glucose donor and EGCG asa glucose acceptor by a glucosyltransferase from Leuconostoc mesenteroides. The pH andtemperature for maximum EGCG-G1 production by the glucosyltransferase were 5.0 and 25C, respectively. The enzyme and substrates concentrations to produce maximum EGCG-G1were 3.78 units/mL, 25 mM sucrose, and 1.5 mM EGCG, respectively. Under these conditions,the glucosyltransferase produced 1.0 mM EGCG-G1 from 1.5 mM EGCG at a reaction time of180 min, corresponding to formation of 67% product on a mole basis.