New Strategy for Quantifying Biological Zinc by a Modified Zinpyr Fluorescence Sensor

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• 作者：Xiao-an Zhang ; Dugan Hayes ; Sarah J. Smith ; Simone Friedle ; Stephen J. Lippard
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：November 26, 2008
• 年：2008
• 卷：130
• 期：47
• 页码：15788-15789
• 全文大小：127K
• 年卷期：v.130,no.47(November 26, 2008)
• ISSN：1520-5126

文摘

Substitution of one pyridine by pyrazine in each DPA appendage of ZP1 leads to a new zinc sensor, ZPP1, with a modified background fluorescence and zinc affinity. ZPP1 exhibits a two-step zinc response during fluorescence titrations, which leads to a new method for zinc quantification. The ability of ZPP1 to image and quantify zinc was demonstrated in pancreatic Min6 cells.