Kinetic Enzymatic Determination of Glycerol in Wine and Beer Using a Sequential Injection System with Spectrophotometric Detection

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• 作者：Hugo M. Oliveira ; Marcela A. Segundo ; Jos&eacute ; L. F. C. Lima ; Viviane Grassi ; and Elias A. G. Zagatto
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：June 14, 2006
• 年：2006
• 卷：54
• 期：12
• 页码：4136 - 4140
• 全文大小：65K
• 年卷期：v.54,no.12(June 14, 2006)
• ISSN：1520-5118

文摘

A sequential injection system for the automatic determination of glycerol in wine and beer wasdeveloped. The method is based on the rate of formation of NADH from the reaction of glycerol andNAD⁺ catalyzed by the enzyme glycerol dehydrogenase in solution. The determination of glycerolwas performed between 0.3 and 3.0 mmol L^-1 (0.028 and 0.276 g L^-1), and good repeatability wasattained (rsd < 3.6%, n = 5) for all samples tested. The determination rate was 54 h^-1, the reagentconsumption was only 0.75 mages/entities/mgr.gif">mol of NAD⁺ and 5.4 ng of enzyme per assay, and the waste productionwas 2.12 mL per assay. Results obtained for samples were in agreement with those obtained withthe batch enzymatic method.Keywords: Glycerol; wine; beer; sequential injection analysis; enzymatic determination; spectrophotometry