High-Throughput Sulfide Sensing with Colorimetric Analysis of Single Au鈥揂g Core鈥揝hell Nanoparticles

详细信息    查看全文

• 作者：Jinrui Hao ; Bin Xiong ; XiaoDong Cheng ; Yan He ; Edward S. Yeung
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：May 20, 2014
• 年：2014
• 卷：86
• 期：10
• 页码：4663-4667
• 全文大小：262K
• 年卷期：v.86,no.10(May 20, 2014)
• ISSN：1520-6882

文摘

We present a high-throughput strategy for sensitive detection of H<sub>2sub>S by using individual spherical Au鈥揂g core鈥搒hell plasmonic nanoparticles (PNPs) as molecular probes. This method is based on quantification of color variation of the single PNPs resulting from formation of Ag<sub>2sub>S on the particle surface. The spectral response range of the 51 nm PNP was specifically designed to match the most sensitive region of color cameras. A high density of immobilized PNPs and rapid color RGB (red/green/blue) analysis allow a large number of individual PNPs to be monitored simultaneously, leading to reliable quantification of color change of the PNPs. A linear logarithmic dependence on sulfide concentrations from 50 nM to 100 渭M was demonstrated by using this colorimetric assay. By designing PNPs with various surface chemistries, similar strategies could be developed to detect other chemically or biologically important molecules.