A microscale thermal field-flow fractionation (
![](/images/entities/mgr.gif)
-TFFF)system has been designed, fabricated, and characterized.Motivation for miniaturization of TFFF systems was established by examining the geometrical scaling of thefundamental TFFF theory. Miniaturization of conventionalmacroscale TFFF systems was made possible throughutilization of micromachining technologies. Fabrication ofthe
![](/images/entities/mgr.gif)
-TFFF system was discussed in detail. The
![](/images/entities/mgr.gif)
-TFFFsystem was characterized for plate height versus flow rate,single-component polystyrene retention, and multicomponent polystyrene separations. Retention, thermal diffusion coefficients, and maximum diameter-based selectivity values were extracted from separation data andfound comparable with macroscale TFFF system results.Retention values ranged from 0.33 to 0.46. Thermaldiffusion coefficients were between 3.0 × 10
-8 and 5.4× 10
-8 cm
2/s·K. The maximum diameter-based selectivitywas 1.40.