An Investigation of Liquid Carryover and Sample Residual for a High-Throughput Flow Cytometer Sample Delivery System
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- 作者：Joseph W. Bartsch ; Hy D. Tran ; Anna Waller ; Andrea A. Mammoli ; Tione Buranda ; Larry A. Sklar ; and Bruce S. Edwards
- 刊名：Analytical Chemistry
- 出版年：2004
- 出版时间：July 1, 2004
- 年：2004
- 卷：76
- 期：13
- 页码：3810 - 3817
- 全文大小：128K
- 年卷期：v.76,no.13(July 1, 2004)
- ISSN：1520-6882
文摘
The phenomenon of intersample contamination in air-segmented continuous-flow assays has been studied formany years, and new uses are being found for thesesampling techniques every day. One application that hasbeen developed recently employs a flow cytometer toconduct high-throughput screening assays of biologicalcompounds. We have explored the sources of intersamplecontamination in the system and shown how methodsdeveloped previously can be applied to describe thesephenomena. Using a simple model, we were able toaccurately measure liquid film thickness in the sampletubing and demonstrate the effects of intersample contamination in a flow cytometer assay. Also, measures havebeen taken to reduce the level of intersample contamination in cytometric screening assays, helping to make thesystem a more viable tool for drug screening applications.