A New HPLC-ELSD Method To Quantify Indican in Polygonum tinctorium L. and To Evaluate -Glucosidase Hydrolysis
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- 作者：Luciana G. Angelini ; Elisabetta Campeol ; Sabrina Tozzi ; Kerry G. Gilbert ; David T. Cooke ; and Philip John
- 刊名：Biotechnology Progress
- 出版年：2003
- 出版时间：December 2003
- 年：2003
- 卷：19
- 期：6
- 页码：1792 - 1797
- 全文大小：99K
- 年卷期：v.19,no.6(December 2003)
- ISSN：1520-6033
文摘
A method to quantify the indigo precursor indican (indoxyl-
-D-glucoside) in Polygonumtinctorium L. has been developed. Plant material was extracted in deionized water,and indican was identified and quantified using high performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector (ELSD). Resultsconfirmed that with this method it is possible to measure indican content in a shorttime, obtaining reliable and reproducible data. Using this method, leaf indican contentwas quantified every 15 days during the growing season (from May to October) in P.tinctorium crops grown in a field experiment in Central Italy. Results showed thatindican increased along the growing season until flowering and was positively affectedby photosynthetic active radiation (PAR). Indican is naturally hydrolyzed by native-glucosidase to indoxyl and glucose, the indoxyl yielding indigo. The activity of twoenzymes, sweet almond -glucosidase and Novarom G preparation, were comparedwith P. tinctorium native -glucosidase to evaluate indigo production. Results showedthat the ability to promote indigo formation increased as follows: almond -glucosidase
Novarom G.
-D-glucoside) in Polygonumtinctorium L. has been developed. Plant material was extracted in deionized water,and indican was identified and quantified using high performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector (ELSD). Resultsconfirmed that with this method it is possible to measure indican content in a shorttime, obtaining reliable and reproducible data. Using this method, leaf indican contentwas quantified every 15 days during the growing season (from May to October) in P.tinctorium crops grown in a field experiment in Central Italy. Results showed thatindican increased along the growing season until flowering and was positively affectedby photosynthetic active radiation (PAR). Indican is naturally hydrolyzed by native-glucosidase to indoxyl and glucose, the indoxyl yielding indigo. The activity of twoenzymes, sweet almond -glucosidase and Novarom G preparation, were comparedwith P. tinctorium native -glucosidase to evaluate indigo production. Results showedthat the ability to promote indigo formation increased as follows: almond -glucosidase Novarom G.