Voltammetry Assisted by Multivariate Analysis as a Tool for Speciation of Metallothioneins: Competitive Complexation of - and ![]()
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- 作者：Marí ; a José ; Ló ; pez ; Cristina Ariñ ; o ; Silvia Dí ; az-Cruz ; José ; Manuel Dí ; az-Cruz ; Roman Tauler ; and Miquel Esteban
- 刊名：Environmental Science & Technology
- 出版年：2003
- 出版时间：December 15, 2003
- 年：2003
- 卷：37
- 期：24
- 页码：5609 - 5616
- 全文大小：489K
- 年卷期：v.37,no.24(December 15, 2003)
- ISSN：1520-5851
文摘
Multivariate Curve Resolution by Alternating LeastSquares (MCR-ALS) is applied to voltammetric dataobtained in the study of competitive complexation of Zn(II)and Cd(II) by 
- and 
-domains of metallothionein (MT).The application of MCR-ALS allows the estimation of boththe voltammograms and the concentration profilesassociated with each electrochemical process. Thecomplexity of the voltammograms obtained in titrations ofthe - or -domains of MT with Cd(II) and later Zn(II)(or vice versa) prevents their direct interpretation usingtraditional electrochemical methods. But when MCR-ALSis applied, voltammograms can be interpreted rathersatisfactorily in both qualitative and quantitative terms. MCR-ALS showed the formation of Cd2Zndom and Cd3Zndomcomplexes when both metals were competitively added.A method based on the combined use of voltammetry andsome chemometric techniques is proposed. It can beuseful for the metal speciation of environmentally relevantnatural ligands.
- and -domains of metallothionein (MT).The application of MCR-ALS allows the estimation of boththe voltammograms and the concentration profilesassociated with each electrochemical process. Thecomplexity of the voltammograms obtained in titrations ofthe - or -domains of MT with Cd(II) and later Zn(II)(or vice versa) prevents their direct interpretation usingtraditional electrochemical methods. But when MCR-ALSis applied, voltammograms can be interpreted rathersatisfactorily in both qualitative and quantitative terms. MCR-ALS showed the formation of Cd2Zndom and Cd3Zndomcomplexes when both metals were competitively added.A method based on the combined use of voltammetry andsome chemometric techniques is proposed. It can beuseful for the metal speciation of environmentally relevantnatural ligands.