A Simple and Practical Spreadsheet-Based Method to Extract Single-Molecule Dissociation Kinetics from Variable Loading-Rate Force Spectroscopy Data
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- 作者：Michael J. Serpe ; Farrell R. Kersey ; Jason R. Whitehead ; Scott M. Wilson ; Robert L. Clark ; Stephen L. Craig
- 刊名：Journal of Physical Chemistry C
- 出版年：2008
- 出版时间：December 11, 2008
- 年：2008
- 卷：112
- 期：49
- 页码：19163-19167
- 全文大小：203K
- 年卷期：v.112,no.49(December 11, 2008)
- ISSN：1932-7455
文摘
A simple, practical, and model-free method is presented, by which kinetic data can be extracted from variable loading-rate single molecule dynamic force spectroscopy experiments. The constant sampling rate of digital acquisition facilitates the sorting of multiple force curves into histograms that reflect the collective force−time history of the bonds of interest. Combining the force−time history with similar histograms of force-induced events, in particular bond rupture, gives a direct measure of rate vs applied force. The utility of the method is demonstrated through its application to simulated data.