Identification of Specific Protein Markers in Microdissected Hepatocellular Carcinoma

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• 作者：Christian Melle ; G&uuml ; nther Ernst ; Olaf Scheibner ; Roland Kaufmann ; Bettina Schimmel ; Annett Bleul ; Utz Settmacher ; Merten Hommann ; Uwe Claussen ; Ferdinand von Eggeling
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：January 2007
• 年：2007
• 卷：6
• 期：1
• 页码：306 - 315
• 全文大小：528K
• 年卷期：v.6,no.1(January 2007)
• ISSN：1535-3907

文摘

At present, the molecular mechanisms of hepatocellular carcinogenesis are not well-understood, andhepatocellular carcinoma (HCC) stays one of the most frequent and high-risk metastatic visceralneoplasms worldwide. For the identification of tumor-relevant proteins, we analyzed microdissectedcells from nontumorous liver tissue (n = 28) and tissue derived from hepatic tumor center (n = 25), aswell as tumor margin (n = 23). We unequivocally identified 53 proteins from hepatic tumor tissues bypeptide fingerprint mapping and SELDI mass spectrometry that were separated using two-dimensionalgel electrophoresis. Among a number of signals that were detected as significantly different in theprotein profiling analysis, we identified for the first time ferritin light subunit (FLS) and adenylate kinase3 alpha-like 1 (AK3), showing decreased expressions in hepatic tumor, as well as biliverdin reductaseB (BVRB) that was upregulated in HCC. The use of ProteinChip technology in combination with tissuemicrodissection gives insight of the complex changes occurring at the protein level in hepatocellularcancer associated with tumor development and progression and resulted in three new potentialdiagnostically useful markers.