Selective and Sensitive Detection of Intracellular O2鈥⑩€?/sup> Using Au NPs/Cytochrome c as SERS Nanosensors

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• 作者：Lu-Lu Qu ; Da-Wei Li ; Li-Xia Qin ; Jin Mu ; John S. Fossey ; Yi-Tao Long
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：October 15, 2013
• 年：2013
• 卷：85
• 期：20
• 页码：9549-9555
• 全文大小：468K
• 年卷期：v.85,no.20(October 15, 2013)
• ISSN：1520-6882

文摘

A novel surface-enhanced Raman scattering (SERS) nanosensor was developed by modifying oxidized cytochrome c (Cyt c) on gold nanoparticles (Au NPs) for the sensitive and selective determination of intracellular superoxide anion radical (O₂^{鈥⑩€?/sup>). On the basis of the differences in the SERS spectra between the oxidized and reduced form of Cyt c, this nanosensor could be employed to investigate O₂鈥⑩€?/sup> concentration by measuring the SERS spectra of the reduced Cyt c. Using this SERS nanosensor, a detection limit of 1.0 脳 10鈥? M for O₂鈥⑩€?/sup> could be attained. Additionally, the selectivity of the SERS nanosensor for O₂鈥⑩€?/sup> was examined, showing that other reactive oxygen species (ROS) and biologically relevant species did not influence the detection of O₂鈥⑩€?/sup>. More importantly, the nanosensor could be delivered to the living HeLa and normal human liver cells and permitted the concentration of O₂鈥⑩€?/sup> to be monitored in real time and in a noninvasive manner, which indicates that this nanosensor will be suitable for the qualitative and quantitative analysis of O₂鈥⑩€?/sup> in biosystems, thus leading to a greater understanding of oxidative-stress-related diseases at a cellular level.}