Triacylglycerol Analysis in Human Milk and Other Mammalian Species: Small-Scale Sample Preparation, Characterization, and Statistical Classification Using HPLC-ELSD Profiles

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• 作者：Isabel Ten-Dom茅nech ; Eduardo Beltr谩n-Iturat ; Jos茅 Manuel Herrero-Mart铆nez ; Juan Vicente Sancho-Llopis ; Ernesto Francisco Sim贸-Alfonso
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：June 24, 2015
• 年：2015
• 卷：63
• 期：24
• 页码：5761-5770
• 全文大小：291K
• ISSN：1520-5118

文摘

In this work, a method for the separation of triacylglycerols (TAGs) present in human milk and from other mammalian species by reversed-phase high-performance liquid chromatography using a core鈥搒hell particle packed column with UV and evaporative light-scattering detectors is described. Under optimal conditions, a mobile phase containing acetonitrile/n-pentanol at 10 掳C gave an excellent resolution among more than 50 TAG peaks. A small-scale method for fat extraction in these milks (particularly of interest for human milk samples) using minimal amounts of sample and reagents was also developed. The proposed extraction protocol and the traditional method were compared, giving similar results, with respect to the total fat and relative TAG contents. Finally, a statistical study based on linear discriminant analysis on the TAG composition of different types of milks (human, cow, sheep, and goat) was carried out to differentiate the samples according to their mammalian origin.