Activity-Guided Isolation of Resveratrol Oligomers from a Grapevine-Shoot Extract Using Countercurrent Chromatography
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- 作者：Sebastian Macke ; Gerold Jerz ; Michael T. Empl ; Pablo Steinberg ; Peter Winterhalter
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：December 5, 2012
- 年：2012
- 卷：60
- 期：48
- 页码：11919-11927
- 全文大小：299K
- 年卷期：v.60,no.48(December 5, 2012)
- ISSN：1520-5118
文摘
An activity-guided isolation of bioactive stilbenes has been carried out with the grapevine-shoot extract Vineatrol 30. After hexane precipitation of the polymeric constituents, the stilbene mixture was separated on a preparative scale using low-speed rotary countercurrent chromatography (LSRCCC). The antiproliferative activity of the separated LSRCCC fractions was then screened in the human cancer cell line A-431, and trans-resveratrol, trans-蔚-viniferin, r-2-viniferin, hopeaphenol, and miyabenol C were identified as active principles. In addition, a new class of stilbene derivatives, which exhibit a 纬-lactam ring structure and exert a weak growth-inhibiting activity in A-431 cells, has been identified.