文摘
Several studies have documented the occurrence and fate of trenbolone acetate (TBA) metabolites in soil and water. However, considerable uncertainty still exists with respect to TBA risk in agro-ecosystems because limited data are available to quantify excretion, transformation, and leaching processes. To address these uncertainties, we used experimental mesocosms and a mass balance approach to estimate the TBA metabolite leaching potential from manure excreted by implanted (40 mg TBA, 8 mg 17尾-estradiol) beef cattle. Manure sample analysis indicates that over 113 days, a maximum of 9.3% (3,200 渭g/animal unit [AU]) of the implant dose was excreted as 17伪-trenbolone (17伪-TBOH), and <1% was excreted as 17尾-trenbolone (65 渭g/AU) or trendione (3 渭g/AU). While most (>97%) of the total excreted mass of 17伪-TBOH transforms to uncharacterized products, 0.3鈥?.6% (100鈥?20 渭g/AU) of the implant dose accumulates on land surfaces and is available for subsequent transport. During rainfall or irrigation events, a maximum of 0.005鈥?.06% (1.6鈥?2 渭g/AU 17伪-TBOH) or 0.005鈥?.012% (1.8鈥? 渭g/AU 17伪-TBOH) of the dose leached into runoff, respectively. Leaching potentials peak at 5鈥?0 days postimplantation, suggesting that targeted timing of implantation and irrigation could minimize steroid leaching during rainfall and irrigation events.