Colloid Characterization by Sedimentation Field-Flow Fractionation: Correction for Particle-Wall Interaction
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- 作者：P. Stephen Williams ; Yuehong Xu ; Pierluigi Reschiglian ; and J. Calvin Giddings
- 刊名：Analytical Chemistry
- 出版年：1997
- 出版时间：February 1, 1997
- 年：1997
- 卷：69
- 期：3
- 页码：349 - 360
- 全文大小：282K
- 年卷期：v.69,no.3(February 1, 1997)
- ISSN：1520-6882
文摘
The effect of particle-wall interaction on retentionratiosin sedimentation field-flow fractionation (FFF) is shownto be describable in terms of a semiempirical parameter
w having units of length. A method ofexperimentallydetermining the value of this parameter for a given systemis presented. It requires the determination ofretentionratios for a set of particle standards over a range offieldstrengths. The value of w is simply obtained fromtheslope of a certain straight line plot of the retentiondata.Once determined, this parameter may be incorporatedinto the procedure for the determination of particlediameters from measured retention times to take accountof the effects of particle-wall interaction. Inprinciple,w is independent of field strength and is the samefordifferent FFF instruments providing they employ the samecarrier liquid and channel wall material. Therefore,wvalues have the potential to be universal system constantstransferable from one instrument to another.
w having units of length. A method ofexperimentallydetermining the value of this parameter for a given systemis presented. It requires the determination ofretentionratios for a set of particle standards over a range offieldstrengths. The value of w is simply obtained fromtheslope of a certain straight line plot of the retentiondata.Once determined, this parameter may be incorporatedinto the procedure for the determination of particlediameters from measured retention times to take accountof the effects of particle-wall interaction. Inprinciple,w is independent of field strength and is the samefordifferent FFF instruments providing they employ the samecarrier liquid and channel wall material. Therefore,wvalues have the potential to be universal system constantstransferable from one instrument to another.