Analysis of Flavonoids in Propolis and Ginkgo biloba by Micellar Electrokinetic Capillary Chromatography
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- 作者：Liqin Jiang ; Guozhen Fang ; Yan Zhang ; Guojie Cao ; Shuo Wang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2008
- 出版时间：December 24, 2008
- 年：2008
- 卷：56
- 期：24
- 页码：11571-11577
- 全文大小：181K
- 年卷期：v.56,no.24(December 24, 2008)
- ISSN：1520-5118
文摘
A micellar electrokinetic capillary chromatography (MEKC) method has been developed for simultaneous determination of 10 bioactive flavonoids: rutin, apigenin, luteolin, eriodictyol, kaempferol, chrysin, acacetin, flavanone, flavone, and fisetin. The effect of several parameters, such as UV detection wavelength, buffer pH, buffer concentration, sodium dodecyl sulfate (SDS) concentration, β-cyclodextrin (β-CD) concentration, separation voltage, and injection time on the separation of these flavonoids were systematically investigated. The 10 flavonoids were successfully separated within 18 min in 20 mM Na2B4O7−10 mM NaH2PO4 buffer (pH 9.7) containing 100 mM SDS and 16 mM β-CD at a separation voltage of 19 kV, with UV detection at 254 nm. Regression analysis revealed a good linear relationship between the peak area of each analyte and its concentration with detection limits (S/N = 3), ranging from 0.15 to 1.36 μg mL−1. This method could simultaneously quantify the 10 flavonoids and be used in the quality control of functional foods containing propolis and Ginkgo biloba .