Gold Nanoparticle-Graphite-Like C3N4 Nanosheet Nanohybrids Used for Electrochemiluminescent Immunosensor

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• 作者：Lichan Chen ; Xiaoting Zeng ; Peng Si ; Yingmei Chen ; Yuwu Chi ; Dong-Hwan Kim ; Guonan Chen
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：May 6, 2014
• 年：2014
• 卷：86
• 期：9
• 页码：4188-4195
• 全文大小：417K
• 年卷期：v.86,no.9(May 6, 2014)
• ISSN：1520-6882

文摘

Two-dimensional graphite-like carbon nitride nanosheets (g-C₃N₄ NSs) were hybridized with gold nanoparticles (Au NPs) to construct an electrochemiluminescence (ECL) immunosensor. The prepared Au NP-functionalized g-C₃N₄ NS nanohybrids (Au-g-C₃N₄ NHs) exhibit strong and stable cathodic ECL activity compared to g-C₃N₄ NSs due to the important roles of Au NPs in trapping and storing the electrons from the conduction band of g-C₃N₄ NSs, as well as preventing high energy electron-induced passivation of g-C₃N₄ NSs. On the basis of the improved ECL stability and ECL peak intensity of the Au-g-C₃N₄ NHs, a novel ECL immunosensor was developed to detect carcinoembryonic antigen (CEA) as a model target analyte. The ECL immunosensor has a sensitive response to CEA in a linear range of 0.02鈥?0 ng mL^鈥? with a detection limit of 6.8 pg mL^鈥?. Additionally, the proposed immunosensor shows high specificity, good reproducibility, and long-term stability.