Chemical Composition of Five Commercial Gynostemma pentaphyllum Samples and Their Radical Scavenging, Antiproliferative, and Anti-inflammatory Properties
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- 作者：Zhuohong Xie ; Wei Liu ; Haiqiu Huang ; Margaret Slavin ; Yang Zhao ; Monica Whent ; Jessica Blackford ; Herman Lutterodt ; Huiping Zhou ; Pei Chen ; Thomas T. Y. Wang ; Shaoke Wang ; Liangli (Lucy) Yu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：November 10, 2010
- 年：2010
- 卷：58
- 期：21
- 页码：11243-11249
- 全文大小：838K
- 年卷期：v.58,no.21(November 10, 2010)
- ISSN：1520-5118
文摘
Five Gynostemma pentaphyllum (GP) samples were investigated and compared for their chemical compositions and their antioxidant, antiproliferative, and anti-inflammatory effects. Extracts (50% acetone, 75% ethanol, and 100% ethanol) of the five GP samples (GP1−5) differed in their total phenolic, saponin, and flavonoid contents and in their rutin and quercetin concentrations. The highest level of total flavonoids was 63.5 mg of rutin equiv/g in GP4, and the greatest total phenolic content was 44.3 mg of gallic acid equiv/g in GP1 with 50% acetone as the extraction solvent. GP2 had the highest total saponin content of 132.6 mg/g with 100% ethanol as the extraction solvent. These extracts also differed in their scavenging capacity against DPPH and hydroxyl radicals, although they all showed significant radical scavenging capacity. The 100% ethanol extracts also showed dose-dependently strong inhibition on IL-6 and Ptgs2 mRNA expression and weak inhibition on TNF-α mRNA expression. In addition, GP1 had the highest antiproliferative activity at 3.2 mg equiv/mL concentration in HT-29 human colon cancer cells. The results from this study will be used to promote the application of G. pentaphyllum for improving human health.