Antiinflammatory Properties of the Muscadine Grape (Vitis rotundifolia)

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• 作者：Phillip Greenspan ; John D. Bauer ; Stanley H. Pollock ; J. David Gangemi ; Eugene P. Mayer ; Abdul Ghaffar ; James L. Hargrove ; and Diane K. Hartle
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：November 2, 2005
• 年：2005
• 卷：53
• 期：22
• 页码：8481 - 8484
• 全文大小：130K
• 年卷期：v.53,no.22(November 2, 2005)
• ISSN：1520-5118

文摘

The muscadine grape possesses one of the highest antioxidant levels among fruits; yet, the effect ofthis fruit on mammalian metabolic systems has not received significant attention. To examine theantiinflammatory properties of the muscadine, grape skins were dried, pulverized, and extracted (10%w/v) with 50% ethanol. The extract was then tested in two different assays: the release of superoxidein phorbol myristate acetate-activated neutrophils and the release of cytokines [tumor necrosis factor-mages/gifchars/alpha.gif" BORDER=0>(TNF-mages/gifchars/alpha.gif" BORDER=0>), interleukin-1mages/gifchars/beta2.gif" BORDER=0 ALIGN="middle"> (IL-mages/gifchars/beta2.gif" BORDER=0 ALIGN="middle">), and interleukin-6 (IL-6)] by lipopolysaccharide-activated peripheral bloodmononuclear cells. The release of superoxide and cytokines was inhibited by increasing concentrationsof the extract. A 1:100 dilution of the extract inhibited superoxide release by approximately 60%while the release of TNF-mages/gifchars/alpha.gif" BORDER=0> and IL-1mages/gifchars/beta2.gif" BORDER=0 ALIGN="middle"> was reduced at a dilution of 1:200 by approximately 15 and90%, respectively (all P < 0.05). The inhibition pattern on the release of IL-6 was similar to that seenwith TNF-mages/gifchars/alpha.gif" BORDER=0>. In a related in vivo study, rats were fed a diet containing 5% (wt/wt) dried muscadinegrape skins for 14 days and then were injected with carrageenan in the foot pad. After 3 h, pawedema was measured and the rats on the grape skin diet had approximately 50% less paw edemathan controls (P < 0.05). These results demonstrate that the muscadine grape skin powder possessessignificant in vitro and in vivo antiinflammatory properties.Keywords: Muscadine grape (Vitis rotundifolia); superoxide; antiinflammatory; interleukin-1mages/gifchars/beta2.gif" BORDER=0 ALIGN="middle">; interleukin-6; TNF-mages/gifchars/alpha.gif" BORDER=0>; edema