Insights into Redox Partner Interactions and Substrate Binding in Nitrite Reductase from Alcaligenes xylosoxidans: Crystal Structures of the Trp138His and His313Gln Mutants
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- 作者：Mark L. Barrett ; Roger L. Harris ; Svetlana Antonyuk ; Michael A. Hough ; Mark J. Ellis ; Gary Sawers ; Robert R. Eady ; and S. Samar Hasnain
- 刊名：Biochemistry
- 出版年：2004
- 出版时间：December 28, 2004
- 年：2004
- 卷：43
- 期：51
- 页码：16311 - 16319
- 全文大小：656K
- 年卷期：v.43,no.51(December 28, 2004)
- ISSN：1520-4995
文摘
Dissimilatory nitrite reductase catalyses the reduction of nitrite to nitric oxide within the keybiological process of denitrification. We present biochemical and structural results on two key mutants,one postulated to be important for the interaction with the partner protein and the other for substrateentry. Trp138, adjacent to one of the type-1 Cu ligands, is one of the residues surrounding a small depressionspeculated to be important in complex formation with the physiological redox partners, azurin I and II.Our data reveal that the Trp138His mutant is fully active using methyl viologen as an artificial electrondonor, but there is a large decrease in activity using azurin I. These observations together with its crystalstructure at a high resolution of 1.6 Å confirm the importance of Trp138 in electron transfer and thus inproductive interaction with azurin. A "hydrophobic pocket" on the protein surface has been identified asthe channel through which nitrite may be guided to the catalytic type-2 Cu site. Glu133 and His313 at theopening of the pocket are conserved among most blue and green copper nitrite reductases (CuNiRs). Thefailure to soak the substrate into our high-resolution crystal form of native and mutant CuNiRs has beenlinked to the observation of an extraneous poly(ethylene glycol) (PEG) molecule interacting with His313.We present the crystal structure of His313Gln and the substrate-bound mutant at high resolutions of 1.65and 1.72 Å, respectively. The observation of the substrate-bound structure for the His313Gln mutant andinhibitory studies with PEG establishes the role of the hydrophobic pocket as the port of substrate entry.