Two ginsenoside derivatives (
9,
10) along with 10 known ginsenosides (
1鈥?b>8,
11, and
12) were isolated from BST204, which is a crude ginseng extract fermented by enzyme and acid hydrolysis. The two ginsenosides were determined as 12尾,20(
S),25-trihydroxydammara-3-
O-尾-
d-glucopyranoside (
9) and 12尾,20(
R),25-trihydroxydammara-3-
O-尾-
d-glucopyranoside (
10). Compounds
1鈥?b>12 were categorized into stereoisomeric pairs differentiated by
R- or
S-configuration at C-20, the number or position of sugar residues at C-3 or C-6, and the type of derivative at C-21. Their structure鈥揳ctivity relationship was evaluated by the cell viability assay using HSC-T6 cells. Results showed that 20(
S) (
3 >
4,
7 >
8, and
9 >
10), a 2-hydroxy-2-methylbutyl moiety at C-21 (
3,
7 >
9), and the number of sugar residues at C-3 (
3 >
7) significantly affected the antiproliferative activity on HSC-T6 cells. The inhibition of the cell proliferation of compound
3 was assessed by annexin-V/PI staining analysis using flow cytometry.
Keywords:
ginsenosides; antiproliferative; HSC-T6 cells; Panax ginseng