Preparation of three novel dietary fibers (DFs) from mushroom sclerotia, namely,
Pleurotus tuber-
regium,
Polyporous rhinocerus, and
Wolfiporia cocos, by a scale-up modified AOAC procedure usingindustrial enzymes was investigated. A remar
kably high level of total dietary fiber (TDF) ranging from81.7 to 96.3% sample dry matter (DM), in which a content of nonstarch polysaccharide (NSP) rangingfrom 86.6 to 94.3% sclerotial TDF DM, was obtained from the three sclerotia. All sclerotial DFs wererich in
-glucan (the glucose residue ranged from 89.7 to 94.5% NSP DM) with a very low level ofresistant glycogen (ranged from 3.77 to 3.94% sclerotial TDF DM). All three novel sclerotial DFsalso exhibited similar, if not better, physico
chemical and functional properties (pH, color, water bindingcapacity, oil holding capacity, and emulsifying properties) as those of barely DF control and commercialDF-rich ingredients. The potential use of the three mushroom sclerotial DFs as a new
-glucan typeDF-rich ingredient in the food industry was discussed.Keywords: Mushroom sclerotia; total dietary fiber; industrial enzymes; physico
chemical and functionalproperties