Coupling Stimuli-Responsive Magnetic Nanoparticles with Antibody鈥揂ntigen Detection in Immunoassays

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• 作者：Hirokazu Nagaoka ; Yasunobu Sato ; Xiaomao Xie ; Hideyuki Hata ; Masaru Eguchi ; Nobuki Sakurai ; Takeshi Watanabe ; Hiroshi Saitoh ; Akihiko Kondo ; Satoru Sugita ; Noriyuki Ohnishi
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：December 15, 2011
• 年：2011
• 卷：83
• 期：24
• 页码：9197-9200
• 全文大小：758K
• 年卷期：v.83,no.24(December 15, 2011)
• ISSN：1520-6882

文摘

Because current homogeneous immunoassays show some limitations, particularly low sensitivity, we developed a new immunoassay to overcome these limitations. The approach was based on magnetic nanoparticles with a thermoresponsive polymer layer, a negatively charged polymer, and streptavidin鈥揵iotin-based antibody鈥揳ntigen detection and yielded higher sensitivity than commonly used heterogeneous immunoassays. Because no special equipment is needed, it can be applied to currently available absorbance-based systems for high-throughput assays.