In Situ Supramolecular Assembly and Modular Modification of Hyaluronic Acid Hydrogels for 3D Cellular Engineering
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- 作者：Kyeng Min Park ; Jeong-A Yang ; Hyuntae Jung ; Junseok Yeom ; Ji Sun Park ; Keun-Hong Park ; Allan S. Hoffman ; Sei Kwang Hahn ; Kimoon Kim
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：April 24, 2012
- 年：2012
- 卷：6
- 期：4
- 页码：2960-2968
- 全文大小：608K
- 年卷期：v.6,no.4(April 24, 2012)
- ISSN：1936-086X
文摘
A facile in situ supramolecular assembly and modular modification of biocompatible hydrogels were demonstrated using cucurbit[6]uril-conjugated hyaluronic acid (CB[6]-HA), diaminohexane-conjugated HA (DAH-HA), and tags-CB[6] for cellular engineering applications. The strong and selective host鈥揼uest interaction between CB[6] and DAH made possible the supramolecular assembly of CB[6]/DAH-HA hydrogels in the presence of cells. Then, the 3D environment of CB[6]/DAH-HA hydrogels was modularly modified by the simple treatment with various multifunctional tags-CB[6]. Furthermore, we could confirm in situ formation of CB[6]/DAH-HA hydrogels under the skin of nude mice by sequential subcutaneous injections of CB[6]-HA and DAH-HA solutions. The fluorescence of modularly modified fluorescein isothiocyanate (FITC)-CB[6] in the hydrogels was maintained for up to 11 days, reflecting the feasibility to deliver the proper cues for cellular proliferation and differentiation in the body. Taken together, CB[6]/DAH-HA hydrogels might be successfully exploited as a 3D artificial extracellular matrix for various tissue engineering applications.