Polysaccharides from Dioscorea batatas Induce Tumor Necrosis Factor-α Secretion via Toll-like Receptor 4-Mediated Protein Kinase Signaling Pathways

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• 作者：Ju-Yun Liu ; Feng-Ling Yang ; Chun-Ping Lu ; Yu-Liang Yang ; Chi-Luan Wen ; Kuo-Feng Hua ; Shih-Hsiung Wu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：November 12, 2008
• 年：2008
• 卷：56
• 期：21
• 页码：9892-9898
• 全文大小：235K
• 年卷期：v.56,no.21(November 12, 2008)
• ISSN：1520-5118

文摘

The bioactive polysaccharides (named ZPF1) from yam (Dioscorea batatas) were chemically determined, suggesting repeating β-1,4-mannan as mainly having a feature of acetylation on C₂−OH and C₃−OH, around 28%. The ZPF1 participated in the stimulation of murine wild-type macrophages predominantly in tumor necrosis factor-α (TNFα). Toll-like receptor 4 is proved to be one of the cellular receptors in ZPF1-mediated TNFα secretion. Reactive oxygen species transmission and PI3-kinase are found necessary for regulating TNFα secretion by ZPF1 stimulation. Moreover, we found that extracellular signal-regulated kinase 1/2, Jun N-terminal kinase 1/2, and p38 mitogen-activated protein kinase play important roles in the regulation of TNFα secretion in ZPF1-stimulated macrophages.