Salt-Gradient Approach for Regulating Capture-to-Translocation Dynamics of DNA with Nanochannel Sensors

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• 作者：Yuhui He ; Makusu Tsutsui ; Ralph H. Scheicher ; Xiang Shui Miao ; Masateru Taniguchi
• 刊名：ACS Sensor
• 出版年：2016
• 出版时间：June 24, 2016
• 年：2016
• 卷：1
• 期：6
• 页码：807-816
• 全文大小：698K
• ISSN：2379-3694

文摘

Understanding the physical mechanisms that govern the ion and fluidic transport in salt-concentration-based nanochannel/nanopore systems is essential for the potential applications in bioanalysis. One central challenge is to interpret the observed four-stage change from osmosis to the reverse one with increasing salt gradient. Here we provide a unified model that outlines the intriguing role of two competing factors, the exclusion- and diffusion-induced electrical potentials. We demonstrate theoretically a direction control of a hydrodynamic flow via the salt gradient. Based on this, we also propose a salt-gradient approach for regulating DNA motion in nanochannels that enables voltage-free single-molecule capture with a significantly low translocation speed. The present method would be used as a useful protocol to overcome the key hurdle of tailoring the capture-to-translocation dynamics of polynucleotides for nanopore sequencing.