Selective Incorporation of Nitrile-Based Infrared Probes into Proteins via Cysteine Alkylation

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• 作者：Hyunil Jo ; Robert M. Culik ; Ivan V. Korendovych ; William F. DeGrado ; Feng Gai
• 刊名：Biochemistry
• 出版年：2010
• 出版时间：December 14, 2010
• 年：2010
• 卷：49
• 期：49
• 页码：10354-10356
• 全文大小：716K
• 年卷期：v.49,no.49(December 14, 2010)
• ISSN：1520-4995

文摘

The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alkylation or arylation reaction.