Higher Ploidy in Saccharomyces cerevisiae Supports Enhanced Hepatitis B Virus S Cloned Gene Expression at the Pilot Scale

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• 作者：Jeffrey Fu ; Charles B. Parker ; Pamela Burke ; Loren D. Schultz ; Donna L. Montgomery ; Beth A. Arnold ; and Kathleen McGinnis
• 刊名：Biotechnology Progress
• 出版年：1996
• 出版时间：February 1996
• 年：1996
• 卷：12
• 期：1
• 页码：145 - 148
• 全文大小：102K
• 年卷期：v.12,no.1(February 1996)
• ISSN：1520-6033

文摘

The effect of host strain ploidy on the production of hepatitis Bsurface antigen (HBsAg)in Saccharomyces cerevisiae was evaluated at the pilot scale(75 L). We found thatthe accumulation of HBsAg normalized to cell protein was 2-fold higherfor the diploidstrain compared to its isogenic haploid. No detectable differencesin many fermentationparameters were observed (e.g., rate of fermentation, growthrate, final cell yield).However, the enhancement of productivity in the diploid strainappeared to beassociated with a slower rate of plasmid shedding (2 ages/entities/mgr.gif">m element) and,thus, a higheraverage copy number (2-fold at stationary phase) compared to those ofthe haploidstrain.