Synthesis and Mass Cytometric Analysis of Lanthanide-Encoded Polyelectrolyte Microgels

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• 作者：Wanjuan Lin ; Xiaomei Ma ; Jieshu Qian ; Ahmed I. Abdelrahman ; Adrienne Halupa ; Vladimir Baranov ; Andrij Pich ; Mitchell A. Winnik
• 刊名：Langmuir
• 出版年：2011
• 出版时间：June 7, 2011
• 年：2011
• 卷：27
• 期：11
• 页码：7265-7275
• 全文大小：1063K
• 年卷期：v.27,no.11(June 7, 2011)
• ISSN：1520-5827

文摘

This article describes the synthesis and characterization of two series of functional polyelectrolyte copolymer microgels intended for bioassays based upon mass cytometry, a technique that detects metals by inductively coupled plasma mass spectrometry (ICP-MS). The microgels were loaded with Eu(III) ions, which were then converted in situ to EuF₃ nanoparticles (NPs). Both types of microgels are based upon copolymers of N-isopropylacrylamide (NIPAm) and methacrylic acid (MAA), poly(NIPAm/VCL/MAA) (VCL = N-vinylcaprolactam, V series), and poly(NIPAm/MAA/PEGMA) (PEGMA = poly(ethylene glycol)methacrylate, PG series). Very specific conditions (full neutralization of the MAA groups) were required to confine the EuF₃ NPs to the core of the microgels. We used mass cytometry to measure the number and the particle-to-particle variation of Eu ions per microgel. By controlling the amount of EuCl₃ added to the neutralized microgels. we could vary the atomic content of individual microgels from ca. 10⁶ to 10⁷ Eu atoms, either in the form of Eu³⁺ ions or EuF₃ NPs. Leaching profiles of Eu ions from the hybrid microgels were measured by traditional ICP-MS.