Nonclassical SNAPFL Analogue as a Cy5 Resonance Energy Transfer Partner

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• 作者：Sung Hoon Kim ; Jillian R. Gunther ; John A. Katzenellenbogen
• 刊名：Organic Letters
• 出版年：2008
• 出版时间：November 6, 2008
• 年：2008
• 卷：10
• 期：21
• 页码：4931-4934
• 全文大小：176K
• 年卷期：v.10,no.21(November 6, 2008)
• ISSN：1523-7052

文摘

We have synthesized a new SNAPFL analogue (1) that exhibits a large Stokes shift (78 nm) (abs. 542 nm, em. 620 nm) and a good quantum yield. Because of the large overlap between the emission spectrum of 1 and the absorption spectrum of Cy5, 1 functions well as a fluorescence donor to Cy5 and has been used in FRET-based experiments using estrogen receptor site-specifically labeled with Cy5 and a receptor ligand conjugated to SNAPFL.