Preparation of Fe3O4@SiO2@Layered Double Hydroxide Core鈥揝hell Microspheres for Magnetic Separation of Proteins

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• 作者：Mingfei Shao ; Fanyu Ning ; Jingwen Zhao ; Min Wei ; David G. Evans ; Xue Duan
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：January 18, 2012
• 年：2012
• 卷：134
• 期：2
• 页码：1071-1077
• 全文大小：434K
• 年卷期：v.134,no.2(January 18, 2012)
• ISSN：1520-5126

文摘

Three-component microspheres containing an SiO₂-coated Fe₃O₄ magnetite core and a layered double hydroxide (LDH) nanoplatelet shell have been synthesized via an in situ growth method. The resulting Fe₃O₄@SiO₂@NiAl-LDH microspheres display three-dimensional core鈥搒hell architecture with flowerlike morphology, large surface area (83 m²/g), and uniform mesochannels (4.3 nm). The Ni²⁺ cations in the NiAl-LDH shell provide docking sites for histidine and the materials exhibit excellent performance in the separation of a histidine (His)-tagged green fluorescent protein, with a binding capacity as high as 239 渭g/mg. The microspheres show highly selective adsorption of the His-tagged protein from Escherichia coli lysate, demonstrating their practical applicability. Moreover, the microspheres possess superparamagnetism and high saturation magnetization (36.8 emu/g), which allows them to be easily separated from solution by means of an external magnetic field and subsequently reused. The high stability and selectivity of the Fe₃O₄@SiO₂@NiAl-LDH microspheres for the His-tagged protein were retained over several separation cycles. Therefore, this work provides a promising approach for the design and synthesis of multifunctional LDH microspheres, which can be used for the practical purification of recombinant proteins, as well as having other potential applications in a variety of biomedical fields including drug delivery and biosensors.