Novel Estrogen Receptor-Related Transcripts in Marisa cornuarietis; a Freshwater Snail with Reported Sensitivity to Estrogenic Chemicals
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- 作者：Richard Bannister ; Nicola Beresford ; Denise May ; Edwin J. Routledge ; Susan Jobling ; Mariann Rand-Weaver
- 刊名：Environmental Science & Technology
- 出版年：2007
- 出版时间：April 1, 2007
- 年：2007
- 卷：41
- 期：7
- 页码：2643 - 2650
- 全文大小：631K
- 年卷期：v.41,no.7(April 1, 2007)
- ISSN：1520-5851
文摘
We have isolated novel molluskan steroid receptortranscripts orthologous to vertebrate estrogen receptors(ERs) and estrogen receptor-related receptors (ERRs) fromthe freshwater snail Marisa cornuarietis. Radiolabeledligand binding analyses showed that neither recombinantreceptor protein specifically bound 17
-estradiol overthe range applied (0.3-9.6 nM). These novel receptortranscripts have thus been designated mcER-like and mcERRrespectively. Quantitative PCR revealed mcER-like to beexpressed ubiquitously throughout a range of male and femalestructures studied, including neural and reproductivetissues. Highest absolute levels were seen in the male penis-sheath complex. The mcERR mRNA was also expressedubiquitously throughout all male and female tissues analyzedhere, with very low absolute transcript numbers infemale accessory sex structures compared to othertissues.
-estradiol overthe range applied (0.3-9.6 nM). These novel receptortranscripts have thus been designated mcER-like and mcERRrespectively. Quantitative PCR revealed mcER-like to beexpressed ubiquitously throughout a range of male and femalestructures studied, including neural and reproductivetissues. Highest absolute levels were seen in the male penis-sheath complex. The mcERR mRNA was also expressedubiquitously throughout all male and female tissues analyzedhere, with very low absolute transcript numbers infemale accessory sex structures compared to othertissues.