Label-Free Solution-Based Kinetic Study of Aptamer鈥揝mall Molecule Interactions by Kinetic Capillary Electrophoresis with UV Detection Revealing How Kinetics Control Equilibrium

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• 作者：Jiayin Bao ; Svetlana M. Krylova ; Oren Reinstein ; Philip E. Johnson ; Sergey N. Krylov
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：November 15, 2011
• 年：2011
• 卷：83
• 期：22
• 页码：8387-8390
• 全文大小：724K
• 年卷期：v.83,no.22(November 15, 2011)
• ISSN：1520-6882

文摘

Here we demonstrate a label-free solution-based approach for studying the kinetics of biopolymer鈥搒mall molecule interactions. The approach utilizes kinetic capillary electrophoresis (KCE) separation and UV light absorption detection of the unlabeled small molecule. In this proof-of-concept work, we applied KCE-UV to study kinetics of interaction between a small molecule and a DNA aptamer. From the kinetic analysis of a series of aptamers, we found that dissociation rather than binding controls the stability of the complex. Because of its label-free features and generic nature, KCE-UV promises to become a practical tool for challenging kinetic studies of biopolymer鈥搒mall molecule interactions.