Labeled Protein Recognition at a Membrane Bilayer Interface by Embedded Synthetic Receptors

详细信息    查看全文

• 作者：Yoo-Jin Ghang ; Jonathan J. Lloyd ; Melissa P. Moehlig ; Jessica K. Arguelles ; Magi Mettry ; Xing Zhang ; Ryan R. Julian ; Quan Cheng ; Richard J. Hooley
• 刊名：Langmuir
• 出版年：2014
• 出版时间：September 2, 2014
• 年：2014
• 卷：30
• 期：34
• 页码：10161-10166
• 全文大小：311K
• ISSN：1520-5827

文摘

Self-folding deep cavitands embedded in a supported lipid bilayer are capable of recognizing suitably labeled proteins at the bilayer interface. The addition of a choline derived binding 鈥渉andle鈥?to a number of different proteins allows their selective noncovalent recognition, with association constants on the order of 10⁵ M^鈥?. The proteins are displayed at the water:bilayer interface, and a single binding handle allows recognition of the large, charged protein by a small molecule synthetic receptor via complementary shape and charge interactions.