Upconversion Nanoparticles and Monodispersed Magnetic Polystyrene Microsphere Based Fluorescence Immunoassay for the Detection of Sulfaquinoxaline in Animal-Derived Foods

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• 作者：Gaoshuang Hu ; Wei Sheng ; Yan Zhang ; Junping Wang ; Xuening Wu ; Shuo Wang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：May 18, 2016
• 年：2016
• 卷：64
• 期：19
• 页码：3908-3915
• 全文大小：440K
• 年卷期：0
• ISSN：1520-5118

文摘

A novel fluorescence immunoassay for detecting sulfaquinoxaline (SQX) in animal-derived foods was developed using NaYF₄:Yb/Tm upconversion nanoparticles (UCNPs) conjugated with antibodies as fluorescence signal probes, and monodisperse magnetic polystyrene microspheres (MMPMs) modified with coating antigen as immune-sensing capture probes for trapping and separating the signal probes. Based on a competitive immunoassay format, the detection limit of the proposed method for detecting SQX was 0.1 μg L^–1 in buffer and 0.5 μg kg^–1 in food samples. The recoveries of SQX in spiked samples ranged from 69.80 to 133.00%, with coefficients of variation of 0.24–25.06%. The extraction procedure was fast, simple, and environmentally friendly, requiring no organic solvents. In particular, milk samples can be analyzed directly after simple dilution. This method has appealing properties, such as sensitive fluorescence response, a simple and fast extraction procedure, and environmental friendliness, and could be applied to detecting SQX in animal-derived foods.