An Organophosphonate Strategy for Functionalizing Silicon Photonic Biosensors

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• 作者：Jing Shang ; Fang Cheng ; Manish Dubey ; Justin M. Kaplan ; Meghana Rawal ; Xi Jiang ; David S. Newburg ; Philip A. Sullivan ; Rodrigo B. Andrade ; Daniel M. Ratner
• 刊名：Langmuir
• 出版年：2012
• 出版时间：February 14, 2012
• 年：2012
• 卷：28
• 期：6
• 页码：3338-3344
• 全文大小：377K
• 年卷期：v.28,no.6(February 14, 2012)
• ISSN：1520-5827

文摘

Silicon photonic microring resonators have established their potential for label-free and low-cost biosensing applications. However, the long-term performance of this optical sensing platform requires robust surface modification and biofunctionalization. Herein, we demonstrate a conjugation strategy based on an organophosphonate surface coating and vinyl sulfone linker to biofunctionalize silicon resonators for biomolecular sensing. To validate this method, a series of glycans, including carbohydrates and glycoconjugates, were immobilized on divinyl sulfone (DVS)/organophosphonate-modified microrings and used to characterize carbohydrate鈥損rotein and norovirus particle interactions. This biofunctional platform was able to orthogonally detect multiple specific carbohydrate鈥損rotein interactions simultaneously. Additionally, the platform was capable of reproducible binding after multiple regenerations by high-salt, high-pH, or low-pH solutions and after 1 month storage in ambient conditions. This remarkable stability and durability of the organophosphonate immobilization strategy will facilitate the application of silicon microring resonators in various sensing conditions, prolong their lifetime, and minimize the cost for storage and delivery; these characteristics are requisite for developing biosensors for point-of-care and distributed diagnostics and other biomedical applications. In addition, the platform demonstrated its ability to characterize carbohydrate-mediated host鈥搗irus interactions, providing a facile method for discovering new antiviral agents to prevent infectious disease.